Panobinostat affects activity and expression of DNA methyltransferases in vitro . (A) Total DNMT activity was evaluated in HepG2 and Hep3B cells treated with 0.1 μM panobinostat for the indicated points in time. Results are the mean remaining DNMT activity ± relative error of three independent experiments and are expressed relative to values of untreated controls with a set value of 1.0 for each point in time. * P < 0.05 vs. untreated controls. (B) and (C) Quantitative RT-PCR analysis of expression of DNMTs in HepG2 (B) and Hep3B (C) cells after treatment with 0.1 μM panobinostat. Results were normalized to the GAPDH level of each sample and represent mean ± relative error of three independent experiments and are expressed relative to mRNA levels of untreated controls at each point in time using the set value 1.0.