Figure 7

Relative DNA binding affinity of doxorubicin and doxorubicinol measured by a FID assay. Relative DNA binding affinity of doxorubicin and doxorubicinol was measured by adding consecutive aliquots of either doxorubicin or doxorubicinol to ethidium bromide-saturated salmon sperm DNA. Addition of the doxorubicin or doxorubicinol displaced the ethidium bromide and resulted in a progressive net decrease in fluorescence at excitation 545 nm and emission 595 nm.