Elevated expression of miR-21 in human glioblastoma cell lines and down regulation of SOX2 upon miR-21 knock down. A) Human glioblastoma cell lines revealed a higher expression of miR-21 compared to the normal fibroblast cell line, 1064SK, as shown by Northern blot analysis. U6 was used as a loading control. Western blot analysis showing a decreased expression of SOX2, after LNA-miR-21 in the human glioblastoma cell lines U2987MG and Cl2:6 (B), and in human glioblastoma primary culture, U3001MG (C). GAPDH was used as a loading control.