Figure 9
Reovirus induced cell death is not apoptotic in SCCHN cells. A. SCCHN cells shown were infected with reovirus (MOI 1) using viral stocks at 3.7×109 TCID50/ml and Jurkat cells were treated with 10 μM camptothecin (campto). Lysates were resolved on 10% NuPage Novex Bis Tris gels and probed for pro-caspase 3 cleavage. B. Cal27, C. SIHN 5B, D. HN3, E. HN5 and F. 011A were treated with pan-caspase inhibitor (ZVAD, 200 μM) for 2 hr prior to either TNFα-related apoptosis inducing ligand treatment (TRAIL 100ng/ml) or reovirus infection (MOI 10) using viral stocks at 1.9 × 109 TCID50/ml. Cell survival was assessed by MTT assay at 96 hours post-infection. Means are from at least 3 independent experiments and error bars represent SEMs.