Characterization of genetic rearrangements in esophageal squamous carcinoma cell lines by a combination of M-FISH and array-CGH: further confirmation of some split genomic regions in primary tumors

Table 6 Logistic regression analyses of the impact of clinico-pathological factors and 11q13.3-q13.4 splitting and amplification on LNM

Clinical features	LNM		Univariate analysis		Multivariate analysis
	N0	N1	P value	RR (95% CI)	P value^a	RR (95% CI)
Gender
Male	48	61	0.368	1.466 (0.637-3.374)	-
Female	15	13
Age
< 60	32	38	0.948	0.978 (0.499-1.915)	-
≥ 60	31	36
Tumor size
T1, T2	11	9	0.384	1.528 (0.589-3.964)	-
T3, T4	52	65
Differentiation
G1	16	11	0.295	1.308 (0.792-2.161)	-
G2	31	43
G3	16	20
Splitting status
Non-splitting	44	32	0.004	2.822 (1.384-5.757)	0.026	2.357 (1.110-5.004)
Splitting	19	39
Amplification status
Non-amp	13	5	0.022	3.588 (1.202-10.712)	0.165	2.265 (0.715-7.175)
Amp	50	69

^a The P value of each variable which is not significantly correlated with LNM in univariate analysis is indicated with “-” in the multivariate analysis. Multivariate logistic regression analysis is performed using forward procedures.
LNM: lymph node metastasis; RR, relative risk; CI, confidence interval; amp: amplification.