TGF-β induces trans-differentiation in F9-and NMuMG-cells in vitro. (A) Relative gene expression (mRNA levels) of Egf, Tgf-β1 and Tgf-β2 in clodrolip treated vs. control tumors; n = 5-6, bars: means ± SEM. (B) Relative gene expression (mRNA levels) of Csfr-1, Egf, Tgf-β1, and Tgf-β2 in size matched clodrolip treated vs. control tumors; n = 5-6, bars: means ± SEM. The table shows individual volumes and gene expression levels of paired treated vs. control tumors. The tumors analyzed in this figure were collected from a single depletion experiment. (C) Immunofluorescence of E-cadherin, β-catenin, vimentin and fibronectin expression and cellular localization in F9-cells cultured in DMEM +/-EGF (50 ng/ml) and +/- TGF-β1 (2 ng/ml) for 7d. Pictures of NMuMG-cells are shown in Additional file 2: Figure S4. Scale bar = 0.02 mm. Proteins are colored red and nuclei were stained with DAPI (blue). Arrows indicate the location of the annotated protein in cells cultured in DMEM + rTGF-β1, 7d. (D) Relative luciferase expression (TOPFLASH/FOPFLASH) in F9-cells (left) and NMuMG-cells (right) after 7d or 13d of culture, in DMEM ± rEGF (50 ng/ml) or rTGF-β1 (2 ng/ml), respectively.