Figure 2
Expression of versican G3 domain enhanced tumor cell migration and invasion to bone stromal cells and MC3T3-E1 cells. (a) After culture the bone stromal cells or MC3T3-E1 cells in the bottom well of Transwell migration chambers for 12 h, vector- and G3- transfected 66c14 cells (1 × 105) were loaded in the insert with 100 μl serum free DMEM medium and then incubated at 37°C for 4 hours. The migration cells were stained blue and were counted in 6 fields of views/membrane using a light microscope. Typical pictures showed that migration cells of vector- and G- transfected 66c14 cell migration to bone stromal cells and MC3T3-E1 cells after 4 hours. (b) Graph showed vector- and G3- transfected 66c14 cell migration to stromal cells and MC3T3-E1 cells. **, p<0.01. Error bars indicate SD (n = 6). (c) After culture the bone stromal cells or MC3T3-E1 cells in the bottom well of Transwell migration chambers for 12 h, vector- and G3- transfected 66c14 cells (1 × 105) were loaded in the insert pre-set with Matrigel gel with 100 μl serum free DMEM medium and then incubated at 37°C for 48 hours. Graph showed vector- and G3- transfected 66c14 cell invading to stromal cells and MC3T3-E1 cells. **, p<0.01. Error bars indicate SD (n = 6). (d) Typical pictures showed that invasive cells of vector- and G- transfected 4T07 to bone stromal cells and MC3T3-E1 cells. (e) Graph showed that vector- and G3- transfected 66c14 cell invasion to MC3T3-E1 cells which were cultured in selective EGFR inhibitor AG1478 (2 μM), selective MEK inhibitor PD 98059 (50 μM), selective JNK inhibitor SP 600125 (100 nM), or selective AKT inhibitor Triciribine (2 μM). The invasive cells were stained blue and were counted in 6 fields of views/membrane using a light microscope. **, p<0.01. Error bars indicate SD (n = 6).