Fluorescent immuno-histochemical staining of Bcl-2 using the HistoRx
AQUA platform: (A) Representative examples of quantitative fluorescent IHC images for Bcl-2 expression in normal OCSE (top panel) and OSCC (two bottom panels). AQUA scores represent the expression level of Bcl-2 within the pan-cytokeratin defined epithelial/tumour compartment. DAPI-stained nuclei are depicted in blue, pan-cytokeratin-stained epithelial/tumour cells are depicted in green, and Bcl-2 protein expression is depicted in red. (B) Histogram distribution representing Bcl-2 expression within OSCC tumour samples. The solid blue line represents median Bcl-2 expression in normal OCSE, the broken blue lines represent +/−2 standard deviations from median Bcl-2 expression in normal OCSE, and the solid red line represents median Bcl-2 expression in OSCC.