Figure 5

ALDH activity in cancer cells under different culture growing conditions. Aldefluor® was used to estimate ALDH enzyme activity in cells grown as a monolayer culture or in a sphere culture. Flow cytometric graphs show the fluorescence intensity of reacted ALDH substrate in the absence and presence of diethylaminobenzaldehyde, a specific ALDH inhibitor, for A: endometrioid cancer cell line TOV112D; B: Clear cell cancer cell line RMG1; C. Mucinous cancer cell line RMUGL, and D: high-grade serous cancer cells isolated from clinical ascites. Gated regions indicated ALDH⁺ cells.