β-catenin expression levels in K562 and Lucena cells. (A) RT-qPCR analysis of β-catenin mRNA levels. Raw expression values were normalized to β-actin expression. (B) β-catenin expression by FACS, represented as MRFI. Secondary antibody was used as isotype antibody control. (C) Representative western blot analysis of β-catenin expression. 50 μg of protein extracts from both cell lines were separated SDS-PAGE and probed with anti- β-catenin antibody. α-tubulin was used for constitutive expression. Values represent the means of three independent determinations ± s.d.