ZIC1 alters cell-cycle distributions by regulation of p21, p27 and cyclin D1 in gastric cancer cells. (A) Cell cycle distributions were detected by flow cytometry analysis in AGS and SGC7901 cells after transient transfection with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector for 24 h. (B) The expression levels of p21, p27 and cyclin D1 were determined by Western blot. β-actin was detected as a loading control. Densitometry values are expressed as fold change compared with pCDNA3.1 vector control values normalized to 1. (C) The expression levels of phospholated Akt and Erk1/2, as well as total Akt and Erk1/2 were examined by Western blot.