Localization of hornerin fragments in breast cancer cells. (A) Representative western blot of hornerin products in breast cancer cell lines. ER = estrogen receptor, PR = progesterone receptor; α-tubulin was used as a loading control. (B) Representative western blot of MCF10AI subcellular fractionated products. N = nuclear, C = cytoplasmic, M = membrane fraction. Lamin A/C indicates purity of subcellular fractions. (C) Confocal microscopy images of hornerin localization using antibodies directed against the proteins N-terminus and C-terminus in the indicated breast cancer cell lines (63x magnification). For each cell line, the top panel shows hornerin staining (green), while the bottom panel shows both the nuclear (DNA stained with DAPI, blue) and hornerin staining (green).