Figure 7

Metastasis is suppressed through reconstitution of TGFβ signaling. (A) Fluorescent images of primary tumors and the liver from CBS-RII orthotopically implanted animals. Green fluorescence reflects metastatic colony formation from the primary tumor. (B) Fluorescent images of primary tumors and the liver from CBS orthotopically implanted animals. Green fluorescence reflects robust metastatic colony formation from the primary tumor. (C-D) Primary tumors established in animals orthotopically implanted with CBS-RII and CBS tumors were processed for KI-67 and TUNEL staining. Both KI-67 and TUNEL images were captured at 200x magnification. (E-F) Primary tumors formed by CBS-RII and CBS cells were analyzed to evaluate the proliferation and apoptotic rates. Image J software was employed to quantify positive staining cells and total number of cells. Statistical significance was determined using two tailed student’s t test with p value less than 0.0002.