Table 1 Primer sequences

Housekeeping genes

ACTB

actin, beta

F: 5'-AACTGGAACGGTGAAGGTGAC-3'

R: 5'-GACTTCCTGTAACAACGCATCTC-3'

HPRT1

hypoxanthine phosphoribosyltransferase 1

F: 5'-ACATCTGGAGTCCTATTGACATCG-3'

R: 5'-TTAAACAACAATCCGCCCAAAGG-3'

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

F: 5'-GTGAAGGTCGGAGTCAACG-3'

R: 5'-GGTGAAGACGCCAGTGGACTC-3'

TBP

TATA box binding protein

F: 5'-GGTGTTGTGAGAAGATGGATGTTG-3'

R: 5'-CCAGATAGCAGCACGGTATGAG-3'

Target genes

CDKN1A

cyclin-dependent kinase inhibitor 1A (p21, Cip1)

F: 5'-ACTAGGCGGTTGAATGAGAGGTTC-3'

R: 5'-CAGGTCTGAGTGTCCAGGAAAGG-3'

EDN1

endothelin 1

F: 5'-CCAACCATCTTCACTGGCTTCC-3'

R: 5'-GTCAGACACAAACACTCCCTTAGG-3'

EEF1E1

eukaryotic translation elongation factor 1 epsilon 1

F: 5'-TGCGGGAGGTTCTTGTTCTG-3'

R: 5'-CTGTTAGACTTGGACCATTGTTTG-3'

GPR56

G protein-coupled receptor 56

F: 5'-CTACAGCCGAAGAATGTGACTC-3'

R: 5'-GCAGAAGCAGGATGTTTGGG-3'

MRE11A

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

F: 5'-GATGATGAAGTCCGTGAGGCTATG-3'

R: 5'-TGTTGGTTGCTGCTGAGATGC-3'

NFKB1

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

F: 5'-CCGTTGGGAATGGTGAGGTC-3'

R: 5'-TTGAGAATGAAGGTGGATGATTGC-3'

OBFC2B

oligonucleotide/oligosaccharide-binding fold containing 2B

F: 5'-GACGATGTTGGCAATCTG-3'

R: 5'-TGGCTCACTGAAGTTAGG-3'

PML

promyelocytic leukemia

F: 5'-CCAAGGCAGTCTCACCAC-3'

R: 5'-TTCGGCATCTGAGTCTTCC-3'

SOD2

superoxide dismutase 2, mitochondrial

F: 5'-GGTGTCCAAGGCTCAGGTTG-3'

R: 5'-GTGCTCCCACACATCAATCCC-3'