Figure 6

MSeA facilitates vascular normalization by induction of vascular maturation. A. Control. IHC double-staining for both of CD31and α-SMA showed that, the xenograft tumor from the nude mice with no MSeA treatment developed dense microvessels (red) but less surrounding pericytes (brown), indicating less maturation of the microvessels; B. MSeA treatment. The IHC double-staining showed increased presentation of α-SMA marker for pericytes (dark brown) along with the blood vessels of the tumor from the nude mice received oral administration of MSeA at 3 mg/kg/day for 18 days. Scale bar = 50 μm. C. Statistical summary of vascular density index. The Vascular Maturation Index (VMI) for the control group and the MSeA treatment group was 60±3% and 80 ±3%, respectively (p < 0.05)