Figure 8

Effect of chrysin on p21 ^WAF1 promoter acivity and role STAT response element in A375 cells. (A) The representation of p21 promoter with essential motifs such as 6 Sp1 sites (from −1 to −119), STAT binding site (−692/-684), p53 response element (p53 RE) -1376/-1395, -2281/-2262. (B) Representation of wild-type STAT response element (5’-TTCCCGAA-3’) and mutant STAT response element (5’-ATGCGGTA-3’).(C) Graph representing p21 promoter activity in A375 cells transfected with p21 promoter which is followed by treatment with chrysin (40 μM) and TSA (4 μM) for 24 h. Chrysin treated cells showed 5–6 fold increase in promoter activity. TSA treated cells showed only 3-folds increase in promoter activity as observed by normalized luciferase values. β-galactosidase O.D values were used for normalizing the Luciferase values.