Figure 2

Chrysin cause G1 cell cycle arrest. (A) Human melanoma A 375 cells were treated with 40 &120 μM of Chrysin (Chry), 40 μM of Oroxylin A (Oro), Methoxy-chrysin (Meo chry) for 24 h. Cell cycle analysis was performed by flowcytometry after propidium iodide staining. The X-axis (FL-2) shows DNA content, and Y-axis (counts) shows the number of cells presented in each phase. TSA (4 μM) was used as positive control. (B,C &D) The data obtained from FACS analysis for a time period of 24, 48 and 72 h was represented in the form of bar diagram. Each experiment was conducted three times and standard deviations were derived. *** indicates p < 0.001, ** indicates p < 0.01, * indicates p < 0.05. The p-values were derived in compound treated cells when compared with control untreated cells using Graphpad software.