Figure 7

DcR3 modulates response to platinum in ovarian cancer cell lines. A and B, Chronic exposure to DcR3 in culture (12 weeks) resulted in increased resistance to cis-platinum in SKOV-3 and OVCAR-3 cells with a mean of 15% more viable cells across a wide range of very high platinum doses, p < 0.001 by ANOVA. C. Photomicrographs demonstrate that DcR3 exposed SKOV-3 cells remain adherent and alive 48 h after platinum has been removed from the cultures where cell numbers continue to decline after platinum has been removed from the parent SKOV-3 cell line. DcR3 treated cells were 60% viable at 48 h by eosin stain. D. CaOV-3 cells exposed to DcR3 under identical conditions demonstrated increased cytotoxicity in response to platinum with > 25% more cell death even at the lower doses, p < 0.001 by ANOVA. Insets indicate that DcR3 exposure has no significant effects on the growth rate of the cell lines tested.