The synergistic antitumor effects of rapamycin with bortezomib on orthotopic tumor model. (A) Schematic representation of the experimental protocol as described in materials and methods. (B) Body weight was recorded to assess toxicity of treatment. (C) Tumors of mice from each group on 5 wk after tumor implantation. (D) Average tumor volume of each group on 5 wk after tumor implantation. *
P < 0.01, versus control group; **
P < 0.001, versus control group; #
P < 0.05, versus rapamycin treatment group. (E) The representative fluorescence images of pulmonary metastasis (arrows) from each group on 5 wk in HCCLM3-R xenograft model (magnification, ×10). (F) The representative HE staining of pulmonary metastasis (arrows) on 5 wk in HCCLM3-R xenograft model (magnification, ×200).