Figure 8

ROS induce JNK activation. A) Inmunocytochemistry of phospho-JNK (red- rodamine and blue–DAPI) and phospho-c-jun (Green-FITC and blue-DAPI) in control cells, treated with 10 μg/ml Cas III-ia and 10 μg/ml Cas III-ia + 10mM N-acety-L- cysteine (NAC) for 24 h. The figures shown are representative of at least three different experiments for each experimental condition. Original magnification, x20. B) Western blot of phospho-JNK and phospho-c-jun in controls, cells treated with 10 μg/ml Cas III-ia and 10 μg/ml Cas III-ia + 10mM NAC for 24 h. The figures shown are representative of at least three different experiments for each experimental condition.