Figure 5.

Increase in cellular ROS is associated with autophagy and apoptosis induced by Cas III-ia. A) ROS generation was determined in lysed cells obtained from controls and 24-h Cas III-ia-treated C6 cells as described in the Materials and Methods section. Each bar represents the mean ± SD (*P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001) of three independent experiments. B) Effect of NAC on cell death of 24 h Cas III-ia-treated cells in the presence of 10 mM NAC. For cell death assay, the cells were treated with 2.5, 5, 10, 15 and 20 μg/ml Cas III-ia for 25 h and analyzed by flow cytometry with propidium iodide. Data representative of means ± SD (*P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001) of three independent experiments. C) Expression of Beclin 1 and Bax proteins in lysated cells of control and treated cells with 10μg/ml Cas III-ia, 10mM NAC and Cas III-ia + 10 mM NAC were determined by W. blot. The figures shown are representative of at least three different experiments for each experimental condition.