Downregulation of PAX3-FKHR decreases the motility of Rh30 cells. (A) Motility of Rh30 cells control clone (CON) decreases when PAX3-FKHR is either stably knocked down (KD1) or transiently knocked down (CON_PF siRNA). The protein levels of PF were shown below the bar graph. The wound confluence of CON was arbitrarily set as 100%. (B) The decreased cell motility of KD1 can be rescued by transiently expressing pcDNA3-PAX3-FKHR (KD1_pcDNA3-PF) but not by expressing an empty vector (KD1_pcDNA3). The protein levels of PF in KD1 transfected with pcDNA3 or pcDNA3-PF were shown below the bar graph. Rh30 control clone (CON) was shown as a reference. PAX3-FKHR siRNA (PF siRNA) or plasmids (either pcDNA3 or pcDNA3-PF) were transfected into CON (CON_PF siRNA) in (A) or KD1 (pcDNA3 or pcDNA3-PF) in (B) 24 hours before real-time monitoring of wound healing. The data, expressed as % Wound Confluence, represent the quantitation of wound healing 30 hours after the scratch was created. (C) Representative microscopic images taken 30 hours after the scratch was created.