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Figure 1 | BMC Cancer

Figure 1

From: Adeno-associated virus-mediated doxycycline-regulatable TRAIL expression suppresses growth of human breast carcinoma in nude mice

Figure 1

AAV-TRE&AAV-Tet-On system regulates targeted gene expression. HEK293T/17 cells were infected with rAAV-TRE-Luc&AAV-Tet-On or rAAV-TRE-Luc for 48 h, respectively. rAAV-CAG-EGFP were used as positive control. (A) The relative light units (RLU) representing luciferase expression in the cells infected with rAAV-TRE-Luc&AAV-Tet-On/Dox+ were detemined on Modulus Microplate Multimode Reader. (B) The EGFP expression in HEK293T/17 cells infected with rAAV-TRE-EGFP&AAV-Tet-On was observed by fluorescence microscopy (100 × magnification). (C) TRAIL protein expression in HEK293T/17 cells infected with rAAV-TRE-TRAIL&AAV-Tet-On for 48 h was detected by Western blot assay. β-actin was used as loading control. (D) Soluble TRAIL secreted into the medium of HEK293T/17 cells at 96 h after the transduction was determined by ELISA. The cells infected with rAAV-TRE-EGFP&AAV-Tet-On were used as negative control. The cells infected with rAAV-CAG-TRAIL were used as positive control. Dox+, 1 μg/ml doxycycline in cell culture medium. Dox-, without doxycycline in the medium.

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BMC Cancer

ISSN: 1471-2407

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