Figure 7
Reactive oxygen species production in CSC, SAR-OS and MNNG/HOS cells following irradiation. Single-cell suspensions were incubated with the fluorescent dye H2DCF-DA for 15 min in the dark. The levels of DCF fluorescence indicative of ROS generation were monitored within the first 60 min following irradiation (2-20 Gy) using a fluorescence microplate reader (excitation: 498 nm, emission: 530 nm) and normalized to the fluorescence values of sham-irradiated cells. Data represent normalized mean fluorescence intensity ± standard deviation, of three independent experiments performed in triplicate. Irradiation did not generate significant increases in ROS production in CSCs (p > 0.05) in relation to sham-irradiated cells. For adherent cells significant increases (p < 0.05) were observed for doses above 8 Gy.