Effect of the Rac1 chemical inhibitor NSC23677 on the subcellular localization of Rac1, and on cell proliferation. A. Representative images of immunocytochemical analysis for Rac1 in C33A and SiHa cells, treated or not with the Rac1 inhibitor NSC23677. B. Detection of Rac1 in cytoplasmic (C) and nuclear (N) proteins from HaCat, C33A and SiHa cells treated or not with the Rac1 inhibitor NSC23677. Lamin B was used as a nuclear marker and α-tubilin is a cytoplasmic marker. C. Results from crystal violet cell proliferation assays on HaCat, C33A and SiHa cells treated or not with the Rac1 inhibitor NSC23766 for 48 h. Data shown are average optical density values plus standard deviation of experiments performed in triplicate.