Figure 3

Involvement of p27 ^Kip1 -mediated G1/S arrest in the protective effects of resveratrol against MG132-mediated cell death. A, The panel of leukemic cells was treated with 5 μM resveratrol or 5 μM MG132 alone or combination for 24 h and cell cycle distribution was measured using FACS analysis. Representative graph from three independent experiments with similar results was shown. B, K562 cells were treated as A, and Western blot analysis was performed. Representative blot from three independent experiments with similar results was shown. C, K562 cells were transfected with scramble or p27^Kip1 siRNA for 24 h, then treated with 5 μM resveratrol or 5 μM MG132 alone or combination for another 24 h. p27^Kip1 expression levels were investigated using Western blot analysis. Representative blot from three independent experiments with similar results was shown. D, Cells were treated as C, and apoptotic cells were measured using Annexin-FITC/PI double staining followed by FACS analysis. The results are presented as mean of three independent experiments performed in triplicate, and error bars represent standard deviation. *, P < 0.05; **, P < 0.01.