Figure 5
From: Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells
Detailed DNA methylation profile of PXR segment 2. Detailed methylation profile of the PXR gene in the 6 colon cancer cell lines and a cancerous tissue sample (255C) and its paired adjacent normal tissue sample (255N). Methylation of each CpG site was estimated by bisulfite sequencing on 7 or 8 individual DNA strands after subcloning. Open and closed circles represent unmethylated and methylated CpG sites, respectively. A 'TATA' indicates a putative TATA box. A curved arrow indicates a transcription start site. 'HpyCH4IV' indicates restriction site using the COBRA assay.