Figure 2
From: SFRP1 reduction results in an increased sensitivity to TGF-β signaling
TGF-β responsive gene expression is augmented in response to SFRP1 loss. After an overnight treatment with 2.5 ng/ml TGF-β1 (A), or an overnight treatment with 10 μM LY364947 (B), total RNA was isolated from three separate harvests for real-time PCR analysis of β3 Integrin and PAI-1. All real-time PCR results are from two separate experiments performed in triplicate and results were normalized to amplification of β-actin mRNA. Bars represent mean ± SEM and are expressed as fold change with respect to TERT-pSUPER cells. *p < 0.05, **p < 0.01, ***p < 0.001 (significantly different from indicated data set using student's t-test)