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Figure 2 | BMC Cancer

Figure 2

From: MicroRNA-203 inhibits cell proliferation by repressing ΔNp63 expression in human esophageal squamous cell carcinoma

Figure 2

Repressing ΔNp63 expression inhibits proliferation of ESCC cell lines. (A, B) The expression level of ΔNp63 protein was detected by Western Blot at 48 hr posttransfection and normalized to that of β-actin. Results showed that the expression of ΔNp63 protein was significantly decreased in cells transfected with ΔNp63 siRNA as compared to those transfected with control siRNA. (C) The expression level of ΔNp63 mRNA was detected by qRT-PCR at 48 hr posttransfection and normalized to that of GAPDH. Results showed that the expression of ΔNp63 mRNA was significantly decreased in cells transfected with ΔNp63 siRNA as compared to those transfected with control siRNA. (D-G) Cell proliferation was evaluated by cell cycle analysis (D), annexin V-FITC/propidium iodide double staining (E), population doubling time determination (F), and clonogenic assay (G) as described in materials and methods. Results showed that the proliferative capacity of cells transfected with ΔNp63 siRNA was significantly lower than that of cells transfected with control siRNA. Transfection condition: Cells (1 × 106) of Eca109 and TE-1 were transfected with 50 pmol of ΔNp63 siRNA (or control siRNA). Data represent mean ± SEM from 4 independent experiments; *, P < 0.05 by t test. **, P < 0.01 by t test.

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