Synergistic antitumor effect of the combination of AAV/TRAIL and cisplatin in vivo. (A) KB cells were inoculated into the right thighs of BALB/c nude mice. When the tumor size was reached to 60 mm3, the mice were received an intratumoral injection of AAV/TRAIL, intraperitoneal injection of cisplatin and the combination treatment. The tumor volume was measured and calculated. The animals were euthanized on the day 33 post-treatment. The tumor weights were determined (B) and processed for immunohistochemistry analysis (× 200) (C). Values are mean with error bar representing standard deviation of the mean (n = 6). *P < 0.05, **P < 0.01 compared to AAV/null.