Figure 2

Cisplatin exhibits dose-dependent cytotoxicity and augments AAV/EGFP transduction in KB cell line. (A) Treatment with cisplatin (50-5000 ng/mL) for 72 h. (B) Fluorescence microscopy of KB cells without (a) or with treatment of cisplatin for 2 h before (b) and after (c) AAV/EGFP infection. For the competition experiments, KB cells were infected with the virus and incubated for 2 h at 37°C in the presence of 500 IU heparin sulfate (d and e). Values are mean of three independent experiments with error bar representing standard deviation of the mean.