Tyrosine kinase chromosomal translocations mediate distinct and overlapping gene regulation events

BMC Cancer

Table 2 Genes regulated by TEL-PDGFRB.

A. Microarray Analysis
Genes Regulated by TEL-PDGFRB
	Fold-Change	Gene Name
Induced Genes	234.50	Chemokine (C-X-C motif) ligand 10 (Cxcl10)
	103.12	Guanylate nucleotide binding protein 1 (Gbp1)
	64.00	Immune responsive gene 1 (Irg1)
	42.22	Ubiquitin specific protease 18 (Usp18)
	19.00	Signal transducer & activator of transcription 1 (Stat1)
	14.55	Interferon regulatory factor 7 (Irf7)
	7.46	Leukemia inhibitory factor (Lif)
	6.96	Jun oncogene (Jun)
	5.84	Vascular endothelial growth factor A (Vegfa)
Suppressed Genes	2.83	Gelsolin (Gsn)
	2.64	Elk3, member of Ets oncogene family (Elk3)
	2.64	Cyclin I (Ccni)
	2.64	Cyclin-dependent kinase inhibitor 2c (Cdkn2c)
	2.46	Leukotriene A4 hydrolase (Lta4h)
	2.46	Adducin 1, alpha (Add1)
	2.46	Neutrophilic granule protein (Ngp)
	2.46	Eukaryotic elongation factor-2 kinase (Eef2k)
	2.46	EGF-like module containing, mucin-like, hormone receptor-like sequence 1 (Emr1)
B. Validation by Quantitative PCR
Gene Name	Mean Fold-Change	p -Value
Cxcl10	61.55 ± 20.98	0.02
Stat1	2.70 ± 0.45	0.009
Gbp1	83.47 ± 20.89	0.008

(A) Selected genes that were induced or suppressed by TEL-PDGFRB by two-fold or more after 1 week of activation. Genes chosen for validation by Q-PCR are denoted by asterisk. (B) Q-PCR was performed in triplicate using RNA samples extracted from 4-5 distinct cell populations. Mean fold-induction at 1 week compared to the expression at 0 h, and SEM are shown for each gene. A student t-test was performed to obtain p-values for the difference between the mean gene expression level at 0 h and 1 week

ISSN: 1471-2407