NSE expression in human glioma specimens. HE staining and ICH staining for NSE on sections from patient specimens. p1 and p3 belonged to the NSE low expression group while p23 and p25 belonged to the NSE high expression group. p3 was oligoastrocytoma (grade II), p1, p23 and p25 were GBMs. NSE staining existed both in the nucleus and cytoplasma. Arrows represent pseudopalisading cells surrounding areas of necrosis. Scale bars in the low magnification image (10×) = 100 μm, in the high magnification image (40×) = 25 μm.