Decreased expression of 17β-hydroxysteroid dehydrogenase type 1 is associated with DNA hypermethylation in colorectal cancer located in the proximal colon

Agnieszka Anna Rawłuszko; Karolina Horbacka; Piotr Krokowicz; Paweł Piotr Jagodziński

doi:10.1186/1471-2407-11-522

Table 1 HSD17B1 transcript and protein levels in primary cancerous and histopathologically unchanged tissue samples from patient with CRC.

From: Decreased expression of 17β-hydroxysteroid dehydrogenase type 1 is associated with DNA hypermethylation in colorectal cancer located in the proximal colon

n = 52		Primary cancerous tissues		Histopathologically unchanged tissues		P^c
		Median (range)	Mean (± SD)	Median (range)	Mean (± SD)
Age (Years)
<60	10	3.511 (2.109-3.868) ^a	3.282 ± 0.5921 ^a	3.415 (2.726-5.107)^a	3.611 ± 0.6309 ^a	0.2450^a
		2.634 (1.681-3.218)^b	2.579 ± 0.5243^b	2.620 (2.108-3.398)^b	2.719 ± 0.4843^b	0.5423^b
>60	42	3.531 (2.071-5.227) ^a	3.569 ± 0.6649 ^a	3.692 (2.790-5.785)^a	3.834 ± 0.6251^a	0.1087^a
		2.728 (1.728-4.023)^b	2.720 ± 0.4702^b	2.952 (1.364-3.472)^b	2.835 ± 0.4913^b	0.1121^b
Gender
Female	20	3.384 (2.109-4.455) ^a	3.371 ± 0.5676 ^a	3.449 (2.726-5.785)^a	3.719 ± 0.7333^a	0.2340 ^a
		2.793 (1.681-3.218)^b	2.666 ± 0.4767^b	2.811 (2.108-3.472)^b	2.844 ± 0.4065^b	0.2119^b
Male	32	3.571 (2.071-4.653) ^a	3.557 ± 0.6523 ^a	3.857(2.790-5.259) ^a	3.890 ± 0.5855 ^a	0.0388 ^a
		2.702 (1.728-4.023)^b	2.713 ± 0.4941^b	2.962 (1.364-3.449)^b	2.792 ± 0.5395^b	0.2832^b
CRC localization
Proximal colon (cecum to transverse)	20	3.507(2.666-4.455) ^a	3.427 ± 0.4350 ^a	3.874 (3.050-5.785) ^a	4.057 ± 0.7488 ^a	0.0016 ^a
		2.793 (1.681-3.218)^b	2.666 ± 0.4767^b	2.811 (2.108-3.472)^b	2.844 ± 0.4065^b	0.0028^b
Distal colon (splenic flexture to sigmoid)	12	3.214 (2.071-4.313) ^a	3.218 ± 0.7760 ^a	3.449 (2.726-4.587) ^a	3.630 ± 0.6372 ^a	0.1685 ^a
		2.798 (1.772-3.254)^b	2.691 ± 0.4777^b	2.860 (1.364-3.391)^b	2.619 ± 0.6350^b	0.7763^b
Rectum	20	3.544 (2.523-4.653) ^a	3.657 ± 0.6011 ^a	3.564 (3.226-4.862) ^a	3.762 ± 0.500 ^a	0.8839 ^a
		2.951 (1.728-3.398)^b	2.815 ± 0.4436^b	2.988 (2.019-3.383)^b	2.913 ± 0.3871^b	0.5019^b
Histologic grade
G1	4	4.059 (2.362-4.402) ^a	3.721 ± 0.9467 ^a	4.431 (3.123-5.259) ^a	4.311 ± 0.9076 ^a	0.4026 ^a
		2.926 (2.586-3.040)^b	2.870 ± 0.2060^b	2.789 (2.026-3.147)^b	2.688 ± 0.5370^b	0.5506^b
G2	35	3.379 (2.109-4.621) ^a	3.363 ± 0.5385 ^a	3.524 (2.726-5.785) ^a	3.681 ± 0.5619 ^a	0.0335 ^a
		2.647 (1.681-4.023)^b	2.649 ± 0.4993^b	2.933 (2.019-3.472)^b	2.863 ± 0.4114^b	0.0659^b
G3	13	3.875 (2.071-4.839) ^a	3.807 ± 0.7092 ^a	4.043 (2.790-5.227) ^a	4.165 ± 0.7346 ^a	0.2587 ^a
		2.676 (1.845-3.431)^b	2.670 ± 0.5098^b	3.023 (1.364-3.449)^b	2.763 ± 0.6858^b	0.5114^b

The HSD17B1 transcript levels^a were standardized by the geometric mean of PBGD and hMRPL19 cDNA and expressed as decimal logarithm of multiples of these cDNA copies in calibrator. ^b The amount of western blot-detected proteins was presented as the decimal logarithm HSD17B1 to β-actin band optical density ratio. ^c Unpaired, two-tailed t-test or U-Mann-Whitney test.

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