Table 1 Antibodies used in this study
Name | Antigen | Source | Manufacturer |
|---|---|---|---|
Twist | Twist | Rabbit | Cell signaling |
Snail | Snail | Rat | Cell signaling |
N-cadherin | N-cadherin | Mouse | Upstate |
Fibronectin Ab-11 | Fibronectin | Mouse | Neo Markers |
Vimentin Ab-2 | Vimentin | Mouse | Neo Markers |
γ-catenin | γ-catenin | Mouse | BD transduction |
E-cadherin | E-cadherin | Mouse | BD transduction |
ZO-1 | ZO-1 | Rabbit | Abcam |
p-GSK-3β (Ser9) | GSK-3β (Ser9) | Mouse | Upstate |
GSK-3β | GSK-3β | Goat | Santa Cruz |
p-Akt (Ser473) | Akt (Ser473) | Rabbit | Cell signaling |
Akt | Akt1,2,3 | Rabbit | Cell signaling |
p-β-catenin(Ser33/37/Thr41) | β-catenin (Ser33/37/Thr41) | Rabbit | Cell signaling |
β-catenin | β-catenin | Rabbit | Abcam |
β-tubulin | β-tubulin | Mouse | Sigma |
Anti-FLAG M2 | FLAG-tag | Mouse | Sigma |
CD44 | CD44 | Mouse | Cell signaling |
PE-Cy7-CD44 | CD44 | Rat | eBioscience |
PE-CD24 | CD24 | Mouse | eBioscience |