Overexpression of Twist induces EMT in Hela and MCF7 cells. (a) Hela and MCF7 cells and their corresponding Twist-overexpressing stable transfectants were analyzed for the expression of N-cadherin, Vimentin, ZO-1, and β-catenin by immunofluorescent staining. Nuclei were visualized with DAPI staining (blue). Cell morphological changes associated with EMT are shown in the phase contrast images. (b) Expression of E-cadherin, Twist, N-cadherin and vimentin in these cells was analyzed by Western blotting. β-tubulin served as a loading control.