Figure 3

Mechanism of α-TEA and trastuzumab-mediated tumor cell inhibition. (A) MDA-MB-453 tumor cells were treated with Vα-TEA and trastuzumab (or isotype antibody) for 72 hours. Cells were collected and analyzed for apoptosis by PE-Annexin-V stain. Mean frequency ± SD of apoptotic cells (PE-Annexin-V positive) is shown from three independent experiments. (B) MDA-MB-453 tumor cells were treated with Vα-TEA and trastuzumab (or isotype antibody) for 24 hours. Phosphorylated-(Ser473)-AKT, total AKT and GAPDH levels were determined by western immunoblotting. Numbers indicate fold-change over untreated (isotype-treated) cells.