Figure 1

Correlation of GFP gene expression with viral transcription, viral copy number and protein expression in cell lines infected with GLV-1h68. A) HT-29 and GI-101A cells infected with GLV-1h68 at different time points and hybridized to a customized whole genome Vaccinia Virus chip; B, C) Screening by RT-PCR of GFP, IMV and Interf proteins in 46 cell lines infected with GLV-1h68. D) Plaque forming assay of A375, DU-145 and A549 cell lines infected with GLV-1h68 for 8, 20 and 36 hours. E) FACS analysis of GFP expression in 74 cell lines (figure showed only 3 of them) after infection of with GLV-1h68. Infections with GLV-1h68 were performed using multiplicity of infections (MOI) 0.3 and MOI 0.6; an almost perfect correlation (R² = 0.96) between the infectivity index obtained in the two conditions (F).