Table 1 Primer sequences used for real-time PCR
From: Effects of EpCAM overexpression on human breast cancer cell lines
Gene | GenBank Acc. Nr. | sequence (5'to 3') |
|---|---|---|
TBP | NM_003194 | fwd TGCACAGGAGCCAAGAGTGAA |
| Â | Â | rev CACATCACAGCTCCCCACCA |
EpCAM | NM_002354 | fwd CGCAGCTCAGGAAGAATGTG |
| Â | Â | rev TGAAGTACACTGGCATTGACG |
TCF7L2 | NM_030756 | fwd TGCACTGTCCAGAGAAGAGC |
| Â | Â | rev GCTGCTTGTCCCTTTTCCTC |
| Â | Â | fwd TCAATGAATCAGAAACGAATCAA |
| Â | Â | rev CTCTTGGCCGCTTCTTCC |
SFRP1 | NM_003012 | fwd GAGTTTGCACTGAGGATGAAAA |
| Â | Â | rev GCTTCTTCTTCTTGGGGACA |
ITF-2 | NM_001083962 | fwd AGCCATTCTCTTCTGCCAAA |
| Â | NM_003199 | rev CAGGTTCTCATCACCCTCGT |
CCND1 | NM_053056 | fwd GCTGCTCCTGGTGAACAAGC |
| Â | NM_001758 | rev TTCAATGAAATCGTGCGGG |
MYC | NM_002467 | fwd CTACGCAGCGCCTCCCTCCACT |
| Â | Â | rev GGCGCTCCAAGACGTTGTGTGTTC |