Figure 9
From: Effects of EpCAM overexpression on human breast cancer cell lines
Wnt signaling activity assay. Upon activation of Wnt signaling a luciferase emitted bioluminescence signal is measured. A reporter plasmid containing a constitutive active TCF/LEF motif determined the 100% activation value while a mutated motif lacking any consensus site for β-catenin binding represented the background luminescence level. Hs578TEpCAM cells showed only a modest activation, whereas EpCAM overexpression in MDA-MB-231EpCAM could activate Wnt signaling about 20% in comparison to their empty vector controls.