Figure 7
Tumorigenicity assay in nude mice. A. Tumor growth curve in nude mice. After tumor cells were injected subcutanecously into the bilateral hind leg of nude mice, the short and long diameters of the tumors were measured weekly and tumor volumes (cm3) were calculated. B. the nude mice with tumor formations. â‘ indicates the nude mouse injected with ishikawa cells transfected with miR-125b plasmid. â‘¡ indicates the nude mouse injected with ishikawa cells transfected with control vector. â‘¢ indicates the nude mouse injected with ishikawa cells with no treatment. C. Photograph of tumors derived from miR-125b plasmid, control vector, or untransfected ishikawa cells in nude mice. D. Weights of tumors. *P < 0.01 as compared with either no transfection group or control vector group. E. Representative HE staining histopathologic image of tumor tissues in mice. (upper panel). ki67 expression of tumors was detected by immunohistochemical techniques (lower panel).