Skip to main content
Figure 2 | BMC Cancer

Figure 2

From: CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers

Figure 2

Mammosphere propagation of cell lines changed the mRNA and protein expression of CD44 isoforms. A. Breast cancer cell lines grown as monolayer cells or mammospheres were analyzed for mRNA expression of CD44 isoforms using q-RT-PCR. In general, the gene expression of the alternatively spliced variants CD44v2-v10, CD44v3-v10, CD44v8-v10 increased and the expression of CD44S decreased for cells cultured as mammospheres. B. Western blot analysis (CD44 antibody, clone 156-3C11) of the breast cancer cell line JIMT-1 confirmed changes in expression of CD44 variants for cells propagated as mammospheres.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us