FACS analysis of CD4
Fc-stimulated T cells. After magnetic separation of CD4 and CD8 T cells followed by two additional rounds of stimulation with Fc1, the T cells were analyzed for expression of CD4/CD8 and of the indicated intracellular cytokines. A. Fc1 CD8+ T cells B. Fc1 CD4+ T cells. The percentages of positive cells are indicated together with the mean fluorescence intensities. Isotype matched negative controls are indicated by dotted lines.