Analysis of selected CD4
Fc-stimulated T cells. A. Growth of CD4+ and CD8+ T cells stimulated with Fc1 after magnetic separation. Total days in culture are given together with the calculated growth factor accumulated over the culture time starting after magnetic separation. B. ELISpot analysis of CD4+ and CD8+ T cells generated against CD40 Bs/HCT116 fusion clone 1 (Fc1). T cells (1 × 104 cells/well) were stimulated with 2 × 104 CD40 Bs, HCT116 or Fc1 as targets. The number of specific IFN-γ releasing activated T cells (spots) among the total number of T cells analyzed (104) and the standard deviation from three replicate wells are given. C. Cytotoxic activity of CD4+ and CD8+ T cells (CD4+ Tc, CD8+ Tc) generated against CD40 Bs/HCT116 fusion clone 1. Left: Specific reactivity of CD4+ Tc and CD8+ Tc against MSI+ HLA-A02+ colon carcinoma cell line HCT116. Right: Specific lytic potential of CD4+ Tc and CD8+ Tc against the MSI+ HLA-A02+ prostate carcinoma cell line LNCaP. Targets were labelled with [51Cr]-sodium chromate for 1 h. Different effector to target cell ratios (E:T ratio) are shown. All results are displayed as the mean and standard deviation from three replicate wells.