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Table 2 Variants detected by HRM analysis

From: Clinical pharmacogenomic testing of KRAS, BRAF and EGFRmutations by high resolution melting analysis and ultra-deep pyrosequencing

Gene

Nucleotide

Protein

No. detected

Total %

KRAS

35G > A

G12D

17

18.3

 

38G > A

G13D

9

9.7

 

34G > T

G12C

7

7.5

 

35G > C

G12A

2

2.2

 

35G > T

G12V

2

2.2

 

34G > A

G12S

2

2.2

 

Total no. of cases with mutated KRAS

39

41.9

 

Total no. of cases

93

100

BRAF

1799T > A

V600E

7

13.0

 

Total no. of cases with mutated BRAF

7

13.0

 

Total no. of cases

54

100

EGFR

2236-2250del15

delE746-A750

1

2.8

 

2237-2252del16

delE746-T751insA

1

2.8

 

2369C > T, 2573T > G

T790M, L858R

1

2.8

 

2543C > T

P848L

1

2.8

 

Total no. cases with mutated EGFR

4

11.1

 

Total no. of cases*

36

100

  1. * Exon 20 was not analysed in 13 samples.