Figure 4

Migration of transduced cells. (A) Scratch wound migration of Sum102 cells expressing pSiRPG-shRNA4 (white bars), -6 (light gray bars), -7 (dark gray bars) or control vector pSiRPG (black bars). Images were captured using a Nikon eclipse TE300 inverted microscope with a Plan 10x/0.25 NA objective (Nikon) and an attached Nikon DS-5M-L1 Camera system. Results are presented as the average width of the wound (n = 4) + SEM of one representative experiment of two (*p <.05 against pSiRPG). (B) Scratch wound widths after 0, 7.5 and 17 hours in cells expressing shRNA6 against TFPI (left) or pSiRPG control vector (right). Migration of transduced cells was also measured using the modified Boyden method. Cells were incubated for 24/16 hours (Sum102/MDA-MB-231) at 37°C before stained with Calcein AM for 90 minutes. Results are presented as mean relative migration + SEM of three to four independent experiments. (C) Sum102 cells (n ≥ 8, *p = .0143 and **p = .0051 against pSiRPG) and (D) MDA-MB-231 cells (n = 9, *p = .0177 against pSiRPG) with TFPI downregulated. (E) MDA-MB-231 cells with TFPIβ downregulated (n = 9, *p = .0103 and **p < .0027 against pSiRPG).