Methylation status of CpG island A in PET, normal pancreas and cell lines. Panel A shows the methylation level of the 51 CpGs analyzed by pyrosequencing in the 20 matched normal/tumor samples. Numbers on the left refer to normal (N) and tumor (T) samples of the 20 PET listed in Table 1. Each of the 51 CpGs, 17 in the promoter region and 34 in the first exon, is represented by a square. Numbers on top show the location of CpG dinucleotides and transcription start site is indicated (+1). Any CpG is represented by a square that has one of four grey levels according to the proportion of methylation detected, namely white, light grey, dark grey and black indicating a level of methylation of 0-20%, 20-40%, 40-60% and > 60%, respectively. Black lines on top of squares indicate location of qMSP and MSP assays spanning CpGs 3 to 12 and CpGs 21 to 34, respectively. Panel B reports the methylation status of the 51 CpGs in the three indicated PET cell lines (CM, QGP1 and BON), where C and D indicates untreated control cells and cell lines treated with demethylating agent, respectively.