(A) P276 specifically inhibits VEGF mRNA expression under hypoxia in a dose-dependent manner in PC-3 cells. However, HIF-1α mRNA levels remained unchanged. (B) Inhibition of HIF-1α and VEGF expression in PC-3 and DU145 prostate cancer cells by P3155. Cells were treated with P3155 (3 μM) for 8 h at 37°C and stained with mouse antibody against HIF-1α and rabbit antibody against VEGF. Reduced coexpression of HIF-1α and VEGF was observed.