Figure 2

P3155 specifically inhibits HIF-1α protein accumulation under hypoxia. PC-3 cells were treated with hypoxic mimic DFX (60 μmol/L) in the presence and absence of P3155 for 8 h, and then protein extracts were prepared as described in 'Materials and Methods' (A) Downregulation of HIF-1α protein by P3155 under hypoxia in a dose-dependent manner (B) P3155 inhibited hypoxia-mediated HIF-1α accumulation in presence of DFX as well as MG132 (proteasome inhibitor) indicating that it inhibits HIF-1α independent of the proteasome activity.