Parameter | Subgroupsa | Sensitivityb | Specificityb | Positive likelihood ratiob | Negative likelihood ratiob |
---|---|---|---|---|---|
Country of origin | Germany (n = 4) | 32.9% (25.7 to 40.8%; I2 = 77.8%) | 98.7% (93.1 to 100%; I2 = 28.6%) | 6.86 (1.99 to 23.63; I2 = 0) | 0.72 (0.57 to 0.92; I2 = 75.1%) |
 | Italy (n = 3) | 55.0% (44.7 to 65.0%; I2 = 70.0%) | 100% (96.3 to 100%; I2 = 0) | 37.15 (7.53 to 183.47; I2 = 0) | 0.43 (0.26 to 0.71; I2 = 74.7%) |
 | Japan (n = 4) | 27.7% (22.1 to 33.8%; I2 = 64.1%) | 100% (96.1 to 100%; I2 = 0) | 13.79 (3.45 to 55.22; I2 = 0) | 0.74 (0.65 to 0.83; I2 = 47.8%) |
 | USA (n = 3) | 29.6% (21.4 to 38.8%; I2 = 95.4%) | 92.7% (89.5 to 95.1%; I2 = 82.2%) | 3.25 (0.27 to 38.72; I2 = 95.4%) | 0.60 (0.18 to 1.97; I2 = 93.4%) |
 | Other (n = 4) | 40.0% (31.7 to 48.8%; I2 = 95.0%) | 70.0% (57.9 to 80.4%; I2 = 94.2%) | 3.34 (0.11 to 104.66; I2 = 90.6) | 0.55 (0.22 to 1.38; I2 = 89.0%) |
Histologic tumor type | TCC ± other components (n = 11) | 28.9% (25.2 to 32.8%; I2 = 87.5%) | 99.6% (97.6 to 100%; I2 = 0) | 8.42 (3.5 to 20.29; I2 = 15.6%) | 0.71 (0.61 to 0.83; I2 = 82.1%) |
 | Not reported (n = 7) | 53.8% (46.3 to 61.2%; I2 = 85.7%) | 89.9% (86.8 to 92.4%; I2 = 93.7%) | 5.89 (1.61 to 21.62; I2 = 89.2%) | 0.49 (0.28 to 0.85; I2 = 89.2%) |
Sampling time | Pretreatment or ≥7 days post-chemotherapy (n = 11) | 31.0% (27.0 to 35.2%; I2 = 50.0%) | 95.8% (93.8 to 97.4%; I2 = 53.1%) | 11.57 (7.28 to 18.40; I2 = 1.3%) | 0.71 (0.62 to 0.81; I2 = 71.2%) |
 | NR or miscellany (n = 7) | 43.2% (36.9 to 49.7%; I2 = 93.8%) | 83.1% (76.2 to 88.7%; I2 = 93.1%) | 5.69 (1.16 to 27.97; I2 = 86.7%) | 0.52 (0.30 to 0.89; I2 = 94.2%) |
Blood sample volume | ≤7.5 ml (n = 8) | 31.3% (26.6 to 36.4%; I2 = 89.2%) | 88.8% (83.5 to 92.8%; I2 = 92.8%) | 8.16 (1.17 to 56.83; I2 = 85.8%) | 0.64 (0.49 to 0.85; I2 = 89.7%) |
 | > 7.5 ml (n = 10) | 38.4% (33.5 to 43.5%; I2 = 89.9%) | 94.7% (92.4 to 96.5%; I2 = 69.6%) | 7.15 (2.52 to 20.32; I2 = 71.2%) | 0.67 (0.54 to 0.83; I2 = 82.4%) |
Collection of two consecutive blood samples | Yes (n = 6) | 41.2% (35.2 to 47.5%; I2 = 90.3%) | 100% (97.1 to 100%; I2 = 0) | 15.32 (4.91 to 47.84; I2 = 0) | 0.61 (0.46 to 0.81; I2 = 87.6%) |
 | No (n = 12) | 31.6% (27.6 to 36.0%; I2 = 89.0%) | 91.6% (89.0 to 93.7%; I2 = 90.3%) | 5.33 (1.93 to 14.68; I2 = 83.0%) | 0.69 (0.56 to 0.85; I2 = 85.4%) |
Cell separation method | Ficoll-Hypaque centrifugation ± further methods (n = 8) | 34.4% (29.2 to 39.9%; I2 = 90.5%) | 87.9% (82.0 to 92.3%; I2 = 92.5%) | 6.99 (0.88 to 55.73; I2 = 85.9%) | 0.69 (0.56 to 0.84; I2 = 76.8%) |
 | RBC lysis protocols (n = 3) | 35.1% (28.4 to 42.2%; I2 = 87.0%) | 91.9% (82.2 to 97.3%; I2 = 87.9%) | 5.74 (0.50 to 65.75; I2 = 75.8%) | 0.69 (0.52 to 0.91; I2 = 66.4%) |
 | Other protocols or NR (n = 7) | 35.5% (29.5 to 41.9%; I2 = 91.3%) | 95.1% (92.8 to 96.9%; I2 = 56.8%) | 10.77 (4.92 to 23.56; I2 = 19.9%) | 0.57 (0.36 to 0.92; I2 = 94.3%) |
Molecular detection technique | RT-PCR based (including nested RT-PCR; n = 13) | 32.0% (28.3 to 36.0%; I2 = 85.8%) | 91.7% (88.1 to 94.5%; I2 = 90.6%) | 7.75 (2.38 to 25.24; I2 = 80.2%) | 0.70 (0.61 to 0.81; I2 = 81.8%) |
 | Other (n = 5) | 45.9% (37.9 to 54.0%; I2 = 93.7%) | 94.1% (91.3 to 96.3%; I2 = 22.2%) | 7.60 (2.17 to 26.67; I2 = 53.3%) | 0.45 (0.22 to 0.94; I2 = 89.4%) |
Nested RT-PCR method | Yes (n = 9) | 30.5% (26.2 to 35.1%; I2 = 71.6%) | 87.4% (82.1 to 91.6%; I2 = 92.3%) | 5.37 (1.58 to 18.27; I2 = 77.7%) | 0.76 (0.70 to 0.82; I2 = 24.7%) |
 | No (n = 9) | 41.0% (35.5 to 46.6%; I2 = 93.4%) | 95.4% (93.2 to 97.1%; I2 = 52.1%) | 10.08 (3.90 to 26.08; I2 = 43.1%) | 0.49 (0.29 to 0.81; I2 = 94.2%) |
PCR marker usedc | CK-20 (n = 7) | 26.4% (21.7 to 31.6%; I2 = 48.8%) | 85.0% (79.1 to 89.7%; I2 = 92.6%) | 3.38 (0.99 to 11.59; I2 = 74.5%) | 0.80 (0.73 to 0.89; I2 = 43.1%) |
 | UP II (n = 5) | 28.4% (23.4 to 33.9%; I2 = 91.9%) | 92.9% (86.5 to 96.9%; I2 = 78.2%) | 4.15 (1.20 to 14.33; I2 = 62.8%) | 0.75 (0.59 to 0.95; I2 = 85.2%) |
 | EGFR (n = 3) | 60.6% (50.0 to 70.6%; I2 = 34.4%) | 89.2% (81.1 to 94.7%; I2 = 95.7%) | 11.94 (0.03 to 4369.8; I2 = 96.2%) | 0.54 (0.23 to 1.28; I2 = 81.1%) |
 | Other (n = 6) | 36.0% (29.7 to 42.6%; I2 = 69.4%) | 77.7% (69.9 to 84.3%; I2 = 90.2%) | 1.48 (0.47 to 4.65; I2 = 88.0%) | 0.87 (0.56 to 1.35; I2 = 81.7%) |